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  Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions. In order to transfer gene a to an F<sup>-</sup>cell, the sex pilus must be in place for at least ____ minutes. A)  0 B)  6 C)  10 D)  14 E)  25 Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions. In order to transfer gene a to an F-cell, the sex pilus must be in place for at least ____ minutes.


A) 0
B) 6
C) 10
D) 14
E) 25

F) A) and E)
G) All of the above

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During bacterial conjugation, bacteria use a long tubular structure called a(n) ____ to contact each other.


A) metabolic pilus
B) asexual pilus
C) flagellum
D) cilia
E) sex pilus

F) A) and E)
G) C) and D)

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How does a phage transfer DNA from one bacterium to another?


A) Phages form a bridge between bacterial cells that allows DNA to pass from the host cell to the recipient cell.
B) Phages form a bridge between bacterial cells that allows DNA to pass in both directions between the two cells.
C) Phages lyse bacterial cells, releasing bacterial DNA into the environment, which is taken up by other phages for transfer to new cells.
D) When phages assemble in an infected bacterial cell, they may incorporate host DNA; released phages may attach to other cells and inject the bacterial DNA into those cells.
E) Phages attach to receptors on bacterial cells and receive some of the target cells' DNA; the phages release this DNA into the environment to be taken up by other bacterial cells.

F) B) and C)
G) A) and D)

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A colony of mice is treated with a nonvirulent strain of the bacterium Streptococcus pneumoniae . Before treatment, a researcher exposed this nonvirulent strain to heat-killed cells of a virulent strain. What will be the effect on the mouse population?


A) The mice will all remain healthy.
B) Some mice will develop a paralytic condition, but most will remain healthy.
C) Most or all of the mice will develop pneumonia.
D) Some mice will develop pneumonia, but most will remain healthy.
E) The mice will become immune to the bacteria.

F) C) and E)
G) All of the above

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Conjugation, transduction, and transformation are the three main mechanisms for gene transfer between bacteria. Compare these three mechanisms in terms of how DNA is transferred.

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Conjugation involves the transfer of DNA...

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T-even bacteriophages ____.


A) infect E. coli
B) are nonvirulent
C) consist of a head with no tail
D) contain two molecules of double-stranded DNA in their head
E) are temperate bacteriophages that do not have a lytic cycle

F) None of the above
G) B) and E)

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The purpose of the sex pilus is to ____.


A) connect bacterial cells so a cytoplasmic bridge can form for conjugation
B) form a gap junction between bacterial cells for conjugation
C) block cytoplasmic exchange
D) enable bacteriophage infection
E) lyse the cell

F) A) and B)
G) A) and C)

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Hfr cells are bacterial cells that ____.


A) perform meiosis
B) have the F factor integrated into their chromosome
C) maintain the F factor in a plasmid
D) have a low frequency of recombination
E) have received DNA from a bacteriophage

F) None of the above
G) B) and C)

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The core of a virus is a ____ molecule.


A) protein
B) sugar
C) nucleic acid
D) fat
E) phosphate

F) D) and E)
G) C) and E)

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How do the constituents of liquid medium differ from solid, gel-like medium?


A) Liquid medium contains agar, a polysaccharide extracted from algae.
B) Solid medium contains agar, a polysaccharide extracted from algae.
C) Solid medium contains agar, a complex lipid extracted from bacterial membranes.
D) Liquid medium contains more salts, which lower the freezing point.
E) Solid medium contains more fatty acids, which raise the melting point.

F) C) and D)
G) A) and E)

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Match each of the following terms with its correct definition.

Premises
Lytic cycle
Reverse transcriptase
Latent stage
Transduction
Conjugation
Capsid
T-even bacteriophages
Bacteriophage lambda
Temperate phage
Retrovirus
Prophage
Virulent phage
Lysogenic cycle
Responses
Type of virus that kills a host cell in every replication cycle
Type of virus that does not kill a host cell in every replication cycle
E. coli phage.
Virus with an RNA genome that is copied into DNA.
During this process, DNA is transferred from one cell to another through a cytoplasmic bridge.
Enzyme that can make DNA from RNA.
Includes phages such as T2 and T4.
The cycle in which a virulent phage kills the bacterial cell by lysis as the phage is released.
Layer of protein surrounding the viral nucleic acid.
This cycle begins when the lambda phage chromosome integrates into the host cell's DNA by crossing-over. Once integrated, the lambda genes are mostly inactive.
When an animal virus remains in the cell in an inactive form.
Integrated viral DNA.
Transferring DNA using a virus.

Correct Answer

Lytic cycle
Reverse transcriptase
Latent stage
Transduction
Conjugation
Capsid
T-even bacteriophages
Bacteriophage lambda
Temperate phage
Retrovirus
Prophage
Virulent phage
Lysogenic cycle

Prions ____.


A) are more complex than viruses
B) have large DNA genomes
C) cause neurodegenerative disorders in mammals
D) infect plant cells
E) encode their own polymerases for nucleic acid replication

F) A) and E)
G) D) and E)

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  Figure 17.1 Use the accompanying figure . If strains 1 and 2 conjugate and are plated on minimal medium, cells growing on the minimal medium will have the genotype ____. A)  bio- met- thr- leu+ B)  bio+ met+ thr+ leu+ C)  bio+ met- thr- leu+ D)  bio+ met+ thr- leu+ E)  bio- met- thr- leu- Figure 17.1 Use the accompanying figure . If strains 1 and 2 conjugate and are plated on minimal medium, cells growing on the minimal medium will have the genotype ____.


A) bio- met- thr- leu+
B) bio+ met+ thr+ leu+
C) bio+ met- thr- leu+
D) bio+ met+ thr- leu+
E) bio- met- thr- leu-

F) A) and B)
G) A) and E)

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  Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . Suppose that after bacterial conjugation, the recipient cell contained the a and b genes. From this information, what can you conclude? A)  The donor cell was unable to make contact with the recipient cell. B)  The donor cell was F<sup>-</sup>. C)  The donor cell produced an abnormal sex pilus. D)  All of the genes could not fit through the sex pilus at one time. E)  The sex pilus was broken before the rest of the genes could be transferred. Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . Suppose that after bacterial conjugation, the recipient cell contained the a and b genes. From this information, what can you conclude?


A) The donor cell was unable to make contact with the recipient cell.
B) The donor cell was F-.
C) The donor cell produced an abnormal sex pilus.
D) All of the genes could not fit through the sex pilus at one time.
E) The sex pilus was broken before the rest of the genes could be transferred.

F) B) and D)
G) A) and C)

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The results of Lederberg and Tatum's experiments with E. coli auxotrophs indicated that ____.


A) bacteria can be transformed when free DNA is available in their environment
B) bacteria are able to exchange genetic material with eukaryotic organisms
C) bacteria reproduce by sexual rather than asexual reproduction
D) genetic recombination can occur in bacteria
E) genetic recombination does not occur in bacteria since they do not undergo meiosis

F) A) and D)
G) A) and C)

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The series of events from infection of a cell through the release of progeny phages from a lysed cell is called the ____ cycle.


A) lytic
B) lysogenic
C) Krebs
D) citric acid
E) lytic or lysogenic

F) All of the above
G) D) and E)

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Bacteria that undergo natural transformation ____.


A) are very common
B) are strains of E. coli
C) are subjected to electroporation to induce DNA uptake
D) generally have a DNA-binding protein on the exterior of their cell wall
E) integrate foreign DNA into their chromosome through conjugation

F) C) and E)
G) B) and E)

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Arrange the following steps of the HIV life cycle in order: 1.Viral RNAs and proteins assemble into new viral particles that bud from the cell. 2) DNA copy of the viral genome is integrated into the host genome. 3) Viral reverse transcriptase makes a double-stranded DNA copy of the viral RNA genome. 4) Viral particle enters the cell. 5) The provirus is transcribed into viral RNA genomes and mRNAs to make viral proteins.


A) 3,2,5,1,4
B) 2,3,4,5,1
C) 4,2,3,1,5
D) 2,4,3,5,1
E) 4,3,2,5,1

F) B) and D)
G) C) and D)

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Which statement correctly describes horizontal gene transfer?


A) Conjugation, transformation, and transduction are all forms of horizontal gene transfer.
B) Conjugation and transformation are forms of horizontal gene transfer, but transduction is a form of vertical gene transfer.
C) Eukaryote-eukaryote transfers of nuclear genes are most common.
D) Prokaryote-eukaryote transfers of nuclear genes are most common.
E) Partial diploids never result from horizontal gene transfer.

F) A) and C)
G) A) and D)

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  Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . According to the accompanying figure, what is the order in which the genes will be transferred to an F- cell during conjugation? A)  c-d-a-b B)  d-a-b-c C)  b-c-d-a D)  a-b-c-d E)  d-c-a-b Figure 17.2 - The accompanying figure above shows the distances between genes in minutes. Assume that the time listed between two genes corresponds to the transfer time of the first of the two genes in the pair. Use the figure to answer questions . According to the accompanying figure, what is the order in which the genes will be transferred to an F- cell during conjugation?


A) c-d-a-b
B) d-a-b-c
C) b-c-d-a
D) a-b-c-d
E) d-c-a-b

F) All of the above
G) B) and C)

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