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The individuality of an organism is determined by the organism's:


A) DNA nucleotide sequence.
B) Nitrogenous bases.
C) Amino acids.
D) Environment.

E) C) and D)
F) All of the above

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Which statement is NOT true? Few forensic labs do analysis of mtDNA because:


A) Little mtDNA is present in a cell.
B) The analysis procedure is very rigorous.
C) It costs much more than nuclear DNA profiling.
D) Such study takes a long time.

E) None of the above
F) B) and C)

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________ repeats are regions of a chromosome that contain multiple copies of a core DNA sequence arranged in a repeating fashion.

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The Y-STR gene can reveal the sex of the person who contributed a DNA sample.

A) True
B) False

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A typical STR DNA type emanating from a single individual shows a ________ band pattern.


A) One
B) Two
C) Three
D) Zero

E) A) and C)
F) None of the above

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B

The molecular structure of DNA was deduced by:


A) Gregor Mendel.
B) Francis Crick.
C) James Watson.
D) Both B and C

E) All of the above
F) C) and D)

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Which statement is not correct for Y-STRs?


A) Female STRs will not yield a Y-STR profile.
B) Y-STRs can be amplified by PCR.
C) Y-STR types are typically shorter in length as compared to X-STRs.
D) A typical Y-STR pattern has one band.

E) A) and B)
F) A) and C)

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Describe the process of DNA replication.What is the importance of DNA replication?

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Answer (should include points such as): DNA replication begins with the unwinding of the DNA strands in the double helix.Each strand is then exposed to a collection of free nucleotides.The double helix is re-created as the nucleotides are assembled in the proper order (A with T and G with C).The result is the emergence of two identical copies of DNA.DNA replication allows a cell to pass on its genetic identity when it divides.

The technology of DNA typing had its beginnings in 1985 with the work of:


A) Henry Lee.
B) Francis Crick.
C) James Watson.
D) Alec Jeffreys.

E) A) and B)
F) A) and C)

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A ________ is a threadlike structure in the cell nucleus along which the genes are located.

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Which of the following depicts correct base-pairing in DNA?


A) A-U
B) G-A
C) T-A
D) C-T

E) A) and B)
F) A) and C)

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C

Which is an advantage of working with short DNA fragments?


A) They are more stable and less likely to break apart.
B) Their quantity can be greatly amplified by PCR technology.
C) They are less subject to degradation due to adverse environmental conditions.
D) All of the above

E) None of the above
F) A) and B)

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How should blood-containing clothes from a victim be packaged?


A) In an airtight metal container
B) In an airtight clear plastic container
C) In a metal paint can
D) In breathable paper after blood has dried

E) B) and D)
F) A) and B)

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Which statement about tandem repeats is NOT true?


A) More than 30% of the human genome is composed of these repeating units.
B) Their origin is a mystery.
C) It is thought that they may act as spacers between the coded regions of DNA.
D) They are of no forensic interest.

E) A) and B)
F) A) and C)

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PCR is a technique that:


A) Provides a statistical analysis of the nitrogenous-base pairings.
B) Can produce many exact copies of segments of DNA.
C) Produces information regarding the sequence of nitrogenous bases.
D) Virtually eliminates operator error from DNA analysis.

E) A) and B)
F) A) and D)

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Which of the following statements about mitochondrial DNA is incorrect?


A) Mitochondrial DNA is located outside the cell's nucleus.
B) Mitochondrial DNA is constructed in a loop configuration.
C) Many copies of mitochondrial DNA's hypervariable regions are made by PCR.
D) The number of repeat segments found in the hypervariable regions are used to type mitochondrialnDNA.

E) A) and D)
F) A) and C)

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________ is the molecule that carries the body's genetic information.

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________ is the process of joining two complementary strands of DNA to form a double-stranded molecule.

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________ region(s) of mitochondrial DNA have been found to be highly variable in the human population for forensic determinations.


A) One
B) Two
C) Three
D) Four

E) B) and C)
F) A) and D)

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The separation of STRs using capillary electrophoresis:


A) Evolved from the flat-gel electrophoresis approach.
B) Decreases analysis time.
C) Is currently the preferred method.
D) All of the above

E) C) and D)
F) A) and C)

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